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Chicken HMGB1/High mobility group protein B1 ELISA KitBrand |
Leading Biology | Catalog Number |
EK00827E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00827E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (High mobility group protein B1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing High mobility group protein B1 The ELISA analytical biochemical technique of the EK00827E kit is based on High mobility group protein B1 antibody-High mobility group protein B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect High mobility group protein B1 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, High mobility group protein B1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Nucleus Chromosome Cytoplasm Secreted
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Chicken
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.062 ng/mL
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Assay Range |
0.156-10 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
HMGB1
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UniProt ID |
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Function |
Multifunctional redox sensitive protein with various roles in different cellular compartments. Nuclear functions are attributed to fully reduced HGMB1. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA, DNA-containing cruciforms or bent structures, supercoiled DNA and ZDNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Can restructure the canonical nucleosome. Proposed to be an universal biosensor for nucleic acids. May promote inflammatory response to sterile and infectious signals and may be involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm may function as sensor and/or chaperone for immunogenic nucleic acids, and mediate autophagy. May act as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
High mobility group protein 1,HMG-1,HMG1,RCJMB04_15a21
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Images |
Standard curve of the product EK00827E. |
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Specification |
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Quantity |
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