>
Antigen, Antibodies, ELISA, Western Blot >
Immunoassay Kits >
ELISA Kit >
Bovine LEP/Leptin ELISA KitBrand |
Leading Biology | Catalog Number |
EK00825E |
Product Type |
ELISA Kit | Field of Research |
Metabolism |
Product Overview |
EK00825E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Leptin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Leptin The ELISA analytical biochemical technique of the EK00825E kit is based on Leptin antibody-Leptin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Leptin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Leptin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
|
||
Cellular Localization |
Secreted
|
||
Assay Type |
Sandwich
|
||
Detection Method |
Spectrophotometrical
|
||
Reactivity |
Bovine
|
||
Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
|
||
Sensitivity |
0.069 ng/mL
|
||
Assay Range |
0.156-10 ng/mL
|
||
Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
|
||
Other Reagents Required |
Deionized or distilled water
|
||
Gene Name |
LEP
|
||
UniProt ID |
|||
Function |
Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (By similarity). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption (By similarity). Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression. Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic
|
||
Form |
ELISA Kits |
||
Storage & Stability |
Store at +4°C. Please refer to protocols.
|
||
Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
|
||
Synonyms |
Obesity factor,OB,OBS
|
||
Images |
Standard curve of the product EK00825E. |
||
Specification |
|||
Quantity |
|
Select | Brand | Catalog No. | Product Name | Pack Size | Type | Field of Research | Specification | Quantity | Price(USD) | |
1 | Leading Biology | EK00487E | Human DCN/Decorin ELISA Kit | 96T | ELISA Kit |
|
$645.00 | Add Ask | ||
2 | Leading Biology | EK00726E | General HS/Heparan sulfate ELISA Kit | 96T | ELISA Kit |
|
$745.00 | Add Ask | ||
3 | Leading Biology | EK00038E | General E2/Estradiol ELISA Kit | 96T | ELISA Kit |
|
$545.00 | Add Ask | ||
4 | Leading Biology | EK0003L | Exendin-4 (Exenatide) ADA | 96T | ELISA Kit |
|
$895.00 | Add Ask | ||
5 | Leading Biology | EK00003E | General Testosterone/Testosterone ELISA Kit | 96T | ELISA Kit |
|
$545.00 | Add Ask | ||
6 | Leading Biology | EK00793E | General GSH/Glutathione ELISA Kit | 96T | ELISA Kit |
|
$745.00 | Add Ask |
Complete this form and click send to ask us a question, request a quote or simply say hello.
You have 0 item in your cart
You have 0 item in your inquiry list