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Immunoassay Kits >
ELISA Kit >
Human CUBN/Cubilin ELISA KitBrand |
Leading Biology | Catalog Number |
EK00815E |
Product Type |
ELISA Kit | Field of Research |
Metabolism |
Product Overview |
EK00815E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Cubilin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cubilin The ELISA analytical biochemical technique of the EK00815E kit is based on Cubilin antibody-Cubilin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cubilin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Cubilin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Apical cell membrane Peripheral membrane protein Cell membrane Peripheral membrane protein Membrane Coated pit Endosome Lysosome membrane Peripheral membrane protein Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells.
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.081 ng/mL
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Assay Range |
0.156-10 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
CUBN
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MIM |
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UniProt ID |
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Function |
Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
460 kDa receptor,Intestinal intrinsic factor receptor,Intrinsic factor-cobalamin receptor,Intrinsic factor-vitamin B12 receptor,IFCR
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Images |
Standard curve of the product EK00815E. |
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Specification |
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Quantity |
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