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Location: Home > DNA, RNA, PCR & Libraries > DNA/RNA Isolation & Purification > RNA Isolation/Purification Reagents > Cultured cells, bacterial total RNA extraction kit (centrifugal column type)

Cultured cells, bacterial total RNA extraction kit (centrifugal column type)

Catalog # Availability Size / Price Inquiry
MR005-01 50 T / $530

Cultured cells, bacterial total RNA extraction kit (centrifugal column type)

Brand

Leading Biology

Catalog Number

MR005

Product Type

RNA Isolation/Purification Reagents

Field of Research

Product Overview

This product is suitable for extracting high-purity bacterial total RNA from various Gram-negative bacteria and Gram-positive bacteria. The kit is equipped with a unique cleavage system, which releases the RNA from the cells and selectively adsorbs to the silicon matrix membrane in the spin column under high unsalted salt state, and then metabolizes the cell metabolite through the deproteinized solution and the rinse solution. Impurities such as proteins are removed, and RNA is finally eluted from the silicon matrix membrane by RNase-Free Water. This product is convenient and quick to extract RNA, and does not need to use toxic and harmful chemicals such as chloroform and phenol, and has no toxic effect on operators and experimental environments.

Kit Components

Lysate RG 30 ml Deproteinized solution RD 16 ml Washing buffer RW 12 ml RNase-Free-Water: 15 ml DNaseⅠ: 1 ml 10 x DNaseⅠ buffer: 500?l RNase-Free adsorption column R1 (with 2 ml collection tube): 50 RNase-Free filter column RF (with 2 ml collection tube): 50 RNase-Free centrifuge tube (1. 5 ml): 50

Form

Kit

Preparation

Lysozyme, β-mercaptoethanol, absolute ethanol.

Storage & Stability

DNase I, 10 x DNase I Buffer Store at -20 ° C; other solutions are stored at room temperature (15-25 ° C).

Applications

This product is suitable for extracting high-purity bacterial total RNA from various Gram-negative bacteria and Gram-positive bacteria.

Images

Results of RNA agarose gel electrophoresis using our Cultured cells, bacterial total RNA extraction kit (centrifugal column type)

Specification

Quantity

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