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  • ChIP-seq Service
    Introduction ChIP sequencing is a technique which combines chromatin immunoprecipitation (ChIP) assays and sequencing together, it’s a powerful method for identifying genome-wide DNA binding sites for transcription factors and other proteins.  During ChIP-seq analysis, the proteins are cross-linked to their bound DNA by formaldehyde treatment, cells are homogenized, and chromatin is sheared and immunoprecipitated with antibody-bound magnetic beads. The immunoprecipitated DNA is then used as the input for a next-generation sequencing (NGS) library prep protocol, where it is sequenced and analyzed for DNA binding sites. Procedures: 1. Cross-linking: The protein was cross-linked to the chromatin under the effect of formaldehyde. 2. Chromatin fragmentation: Sonication to get the DNA fragment. 3. Immunoprecipitation: collect the wanted DNA. Why Leading Biology? At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible. Working with us, you will get stability, and it means a reliable part...
  • Western Blot Service
    Introduction Western blot is an important technique that frequently used in molecular biology. It could let the researchers able to look at a single protein or a subset of proteins via a high sensitivity antibody binding. Western blot could be used to follow protein phosphorylation or identify known and unknown proteins in complexes. There are three steps in Western blot in order to accomplish the tasks above:  (1). The proteins, cell lysates or tissue lysates were run on SDS-polyacrylamide gels, after this step, the proteins were separated by size.  (2). The fractionated proteins, polypeptides or fragments were transferred onto a PVDF membrane, and (3). Probed with a primary-secondary antibody pair to visualize. Procedures: 1. Extraction of total proteins from tissues/cells. 2. Quantitation of total protein using sensitive, specific and accurate protein assay. 3. Fractionation of protein through large SDS-polyacrylamide gel electrophoresis. 4. Transfer protein onto PVDF membranes. 5. Immunostaining blots with antibodies. 6. Scanning protein bands on X-ray films. ...
  • PCR Array
    Introduction PCR array is also called functional classification chip. It combines the advantages of sensitivity and reliability of Real-time quantitative PCR and multiple genes expression level detection simultaneously of Microarray. It’s the first choice to analyze signal pathways or certain biological functions related genes expression status. PCR array is based on the method of fluorescence quantitative PCR and all primers are tested. It has advantages of simple principles, reliable results and good compatibility. The change of a certain disease, signal pathway or biological function related 84 or 372 important genes expression level could be detected quantitatively on a 96-well or 384-well plate simultaneously(others are comparison sample wells). PCR array could analyze a certain disease, signal pathway or biological process related genes expression quantitatively at one time. The application fields of PCR array is wide, mainly includes tumor, immunity, development, metabolism, stem cell, cell biology, cell signal pathway, cardiovascular disease, miRNA target genes, etc. Species available for testing contain Homosapiens, Mus musculus, Rattus norvegicus ...
  • Chemically Synthesized siRNA
    Introduciton siRNA synthesis chemically is the most simple method to silence genes in organisms or cells. It has the advantages of low cost and high transfection efficiency and it is the best way in RNAi clinical treatment. It has an irreplaceable advantage in drug development. The siRNA products synthesized chemically by us are all purified through HPLC method to remove remaining single-strand RNA completely. Just dilute with matched buffer solution could we carry out following experiments. 1.Normal siRNA Normal siRNA product could act on target gene mRNA directly. Please perform real-time quantitative PCR once you receive the products to ensure the change of target mRNA expression level, and confirm whether the siRNA product is effective. 2.Chemically modified siRNA Chemically modified siRNA could solve the stability problem of siRNA in transfection. We provide multiple siRNA chemical modification, mainly contains 2’-hydroxyl methylation modification, phosphoric acid phosphorothioated oligos, fluorinated modification, etc. 3.Fluorescence-labeled siRNA We have advanced siRNA chemosynthetic core technology ...
  • RNA Synthesis
    Introduction Chemosynthetic RNA is an important research tool and it is widely used in gene function analysis, new therapeutic options development and so on. RNA synthesis services include: normal RNA synthesis, RNA modification and labeling, chimeric DNA (hybrid structure of DNA and RNA), 2’-OMe-RNA and other antisense RNAs synthesis and so on. To ensure the quality of RNA oligo, RNA synthesized by us are all identified through the Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry. We execute the QC standard strictly and do purity analysis on the product through HPLC. RNA modification and labeling Terminal Modified Mode Specification Purification Method Price( Modification+Synthesis) 5’ FAM 5OD HPLC Inquiry 5’ Biotin 5OD HPLC ...
  • Northern Blot Service
    Procedures: 1. Isolation total RNA or mRNA from tissue samples. 2. Fractionation of RNA through agarose gel. 3. Transfer RNA onto a supercharged membrane. 4. Cross-liked RNA by UV light. 5. Hybridized membrane with labeled probes. 6. Visualization of the labeled RNA on X-ray film. Why Leading Biology? At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible. Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process. Working with us, you will get the guaranteed service to accommodate your requirements. · Vigorous quality control system to ensure the required quality and reproducibility · Competitive price with fast turnaround time Contact Information Please obtain a quote before ordering, and refer to the quote number when you place an order. Orders are typically confirmed within 12 hours. Have a Question? Email us info@leadingbiology.com Order Products:&...
  • Southern Blot Service (non-radioactive labeled)
    Introduction Southern blot is a widely used technique in molecular biology for the detection of a specific DNA sequence in samples. It’s frequently used to detect the genome integrity and mutation. In Southern bolt, the DNA will be extracted from testing samples followed by UV quantitation and electrophoresis analysis. After restriction digest with enzymes, DNA samples were applied to an agarose gel, then the fragments of DNA were separated by electrophoresis according to size. The gel then where transferred and UV cross-linked to a membrane. The DNA fragments on the membrane were then hybridized by a Chemiluminescent labeled probe. After hybridization, the labeled fragments will be visualized by X-ray. Procedures: 1. Isolation DNA from samples. 2. Fractionation of DNA through denatured agarose gels. 3. Transfer DNA onto supercharged membranes. 4. Cross-linked DNA by UV light. 5. Hybridized membrane with labeled probes. 6. Visualization of the labeled DNA on X-ray film. Why Leading Biology? At Leading Biology, we custom protein purification design for every single prot...
  • PCR/Q-PCR/RT-PCR Service
    Introduction Polymerase Chain Reaction is a widely used technique used to make millions or billions of copies of a particular DNA region. The PCR could be used to sequence DNA fragments, and further diagnose diseases, identify bacteria and viruses, etc. Fig. 1 Reverse transcription polymerase chain reaction (RT-PCR) Reverse transcription polymerase chain reaction (RT-PCR) is a PCR that is designed to detect and measure RNA. Compare to traditional PCR, RT-PCR first taking RNA and converting the RNA strand into its DNA complement. The new complementary DNA containing the reversed transcription will then be amplified. Fig. 2 Quantitative Real-Time PCR Quantitative PCR (qPCR) is used to detect, characterize and quantify nucleic acids. In contrast to traditional PCR end-point detection, qPCR combines PCR amplification and detection into a single step, which means it collects data as the reaction is proceeding. This eliminates using gel electrophoresis to detect the samples, and make the PCR to be truly quantitative. qPCR provides an accurate as well as fast and sensitive means to quantify the products. Pro...
  • PCR Chips
    Introduction PCR chip is a technology which could improve the PCR method by offers shorter assay time, lower reagent consumption, rapid heating and cooling rates as well as the greater potential of integrating multiple processing modules to reduce size and power consumption. Procedures: 1. RNA extraction from samples. 2. RNA quantitation and quality assessment. 3. cDNA templet preparation 4. Data analysis. Why Leading Biology? At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible. Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process. Working with us, you will get the guaranteed service to accommodate your requirements. · Vigorous quality control system to ensure the required quality and reproducibility · Competitive price with fast turnaround time Contact Information Please obtain a quote before ordering, and refer to the quote number when you place an order. Orders are typically con...
  • Full-length gene synthesis
    Introduction There are two ways to obtain genes. The first is getting nucleic acids (genomic DNA or mRNA) in organisms through PCR and RT-PCR. But some genes couldn’t be obtained because of samples and templates, etc. The second is artificial chemical synthesis. Project Name Gene Length (bp) Exprimental Period Price Normal Gene Synthesis <1,000 5-7 days Inquiry 1,000-2,000 5-7 days Inquiry 2,000-3,000 5-7 days Inquiry Long Segment Gene Synthesis >3,000 5-7 days Inquiry ...
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