Introduction

UPP is an expression cell line which could express foreign protein efficiently. The foreign protein can account for more than 20-30% of the total protein it produced, and its principle is based on an enzyme called Marzf that can hydrolyze host mRNA specifically. This enzyme can specificity recognize the ACA sequence of mRNA, and through codon optimization, remove this sequence from the sequence which encoding exogenous protein, and this could avoid the target protein mRNA having the same fate with the host cell protein mRNA. Under the effect of these enzymes, most of the genetic information of the host genes is shielded, while the transcription and translation system still working, this modification can efficiently express the exogenous protein without other interferences. The high Signal-to-noise ratio (SNR) of this technology made the truly high flux protein production came true.

With the constant improvement of the system and related technology innovation, by now, we can use this system to achieve the preparation of 90% of proteins, and with the optimization of the production process, we can achieve 40 times concentrated fermentation system for some proteins, and the expression of protein takes 97.5% of fermentation generations. Another big advantage of this technique is the removal of protein purification steps, because of its high purity which could meet most basic study requirements including membrane protein structure research.

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Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.

• Innovative configurations of chromatography columns custom tailored to each protein
• Vigorous quality control system to ensure the required quality and reproducibility
• Production capacity of up to tens of grams
• Flexible scale-up protein production
• Competitive price with fast turnaround time

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

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By Phone: 1-661-524(LBI)-0262 (USA)