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Location: Home > Custom Services > Sequencing and Chip Technology > Differential Expression Profiling

Differential Expression Profiling

Date: 2018-01-25 Author: Leading Biology Click: 891

Introduction

In the field of molecular biology, gene expression profiling (DNA chip) is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. Techniques to measure this include DNA microarrays that measure the relative activity of previously identified target genes or sequencing technologies that allow profiling of all active genes.


DNA microarrays can simultaneously measure the expression level of thousands of genes within a particular mRNA sample. Such high-throughput expression profiling can be used to compare the level of gene transcription in clinical conditions in order to: (1). identify diagnostic or prognostic biomarkers; (2). classify diseases; (3). monitor the response to therapy; and (4). understand the mechanisms involved in the genesis of disease processes. For these reasons, DNA microarrays are considered important tools for discovery in clinical medicine.


Steps involved in microarrays are as follows:


Differential Expression Profiling

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