Introduction
Shotgun metagenomic sequencing is a relatively new environmental sequencing approach used to examine thousands of organisms in parallel and comprehensively sample all genes, providing insight into community biodiversity and function. The method enables microbiologists to evaluate bacterial diversity and detect the abundance of microbes in various environments. Shotgun metagenomics also provides a means to study unculturable microorganisms that are otherwise difficult or impossible to analyze.
Metagenome sequencing by NGS involves several distinct steps. Firstly, total DNA is extracted from the sample, after the extraction, the DNA undergoes adapter ligation for final Illumina library preparation. The libraries are analyzed using paired-end reads to maximize coverage of the amplicons. The reads are sorted and assembled into contigs. For optional de novo genome assembly, genome binning is performed with the contigs in order to reconstruct complete genomes and to assign these assembled genomes to the closest possible taxonomy. Functional analysis can be performed additionally to detect open reading frames and associated gene functions.
No | Headline | Click | Author | Date |
1 | Plant and Animal Whole Genome Re-Sequencing | 828 | Leading Biology | 2018-01-26 |
2 | Whole Exome Sequencing | 820 | Leading Biology | 2018-01-26 |
3 | Whole Transcriptome Shotgun Sequencing | 1126 | Leading Biology | 2018-01-26 |
4 | smallRNA/microRNA/circRNA/LncRNA Sequencing | 805 | Leading Biology | 2018-01-26 |
5 | Bacterial Genome Sequencing | 815 | Leading Biology | 2018-01-26 |
6 | Targeted Gene Sequencing | 912 | Leading Biology | 2018-01-26 |