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Location: Home > Custom Services > Molecular Biology Services > Paraffin Embedded Tissue Processing

Paraffin Embedded Tissue Processing

Date: 2018-01-25 Author: Leading Biology Click: 1406

Introdution

Biological samples often need to be solidified to allow fine sectioning. Thin slices improve the access of dyes, probes, and antibodies and reduce the overlay of different cell layers in the z-direction. For light microscopy, paraffin wax is the most frequently used hard matrix for cutting. When sectioned, the paraffin tissue slides can be used for a variety of purposes such as special stains, immunohistochemistry, and in situ hybridization to study morphology, protein expression, DNA aberrations and RNA expression.


The procedures to make Paraffin slides are quite simple: Since paraffin is immiscible with water, the main constituent of tissue, samples need to be dehydrated by progressively more concentrated ethanol baths. This is followed by a clearing agent, usually xylene, to remove the ethanol. Finally, molten paraffin wax infiltrates the sample and replaces the xylene.


Paraffin Embedded Tissue Processing

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