Introduction

The Fluorescent Protein contained vectors can be used as transfection markers and to label living cells. They do not contain an MCS. In these vectors, the fluorescent protein is constitutively expressed and can be detected by fluorescence microscopy or flow cytometry, providing direct visual evidence of transfection/cotransfection or the ability to monitor cells after transplantation experiments in vivo. Transfected cells can also be used in assays that require fluorescent labeling of whole cells. The most widely used Fluorescent protein is Green Fluorescent Protein (GFP), and Red Fluorescent Protein (RFP).

 GFP and RFP are both versatile biological markers for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo, GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm, while RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.

Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

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