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Location: Home > Custom Services > Molecular Biology Services > TALEN Technology

TALEN Technology

Date: 2018-01-25 Author: Leading Biology Click: 1485

Introduction

Transcription activator-like effector nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. Transcription activator-like effectors (TALEs) can be quickly engineered to bind practically any desired DNA sequence. By combining such an engineered TALE with a DNA cleavage domain (which cuts DNA strands), one can engineer restriction enzymes that will specifically cut any desired DNA sequence. When these restriction enzymes are introduced into cells, they can be used for gene editing or for genome editing in situ, a technique known as genome editing with engineered nucleases.


The simple relationship between amino acid sequence and DNA recognition of the TALE binding domain allows for the efficient engineering of proteins. Once the TALEN constructs have been assembled, they are inserted into plasmids; the target cells are then transfected with the plasmids, and the gene products are expressed and enter the nucleus to access the genome. Alternatively, TALEN constructs can be delivered to the cells as mRNAs, which removes the possibility of genomic integration of the TALEN-expressing protein. 


TALEN


Procedures:
1. The target sequence is identified
2. A TALEN sequence which corresponds to the target gene is engineered and inserted into a plasmid.
3. Plasmid is inserted into the target cell.

4. TALEN enters the nucleus, binds and cleaves the target sequence.


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