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SNAI1 Antibody (N-term D24)

Catalog # Availability Size / Price Inquiry
APR04612G 100 μl / $495

SNAI1 Antibody (N-term D24)

Brand

Leading Biology

Catalog Number

APR04612G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SNAI1 Antibody (N-term D24).

Molecular Weight

29083 Da

Cellular Localization

Antigen Cellular Localization: Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs

Host

Rabbit

Species Reactivity

Human

Immunogen

9-39 aa

Target

This SNAI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human SNAI1.

Isotype

Rabbit Ig

Symbol

SNAH

GeneID

UniProt ID

Function

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up- regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.

Summary

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Images

Western blot analysis of SNAI1 (arrow) using rabbit polyclonal hSNAI1-D24 (Cat. APR04612G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SNAI1 gene (Lane 2).

Formalin-fixed and paraffin-embedded human Spleen tissue reacted with SNAI1 antibody (N-term D24) (Cat.APR04612G), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

HepG2 cells were incubated with HGF for the time periods indicated. LiCl and MG132 were added 8 h before lysis of the cells. Snai1 protein levels and beta actin as loading control were analyzed by WB.

Specification

Quantity

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