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ETS1 Antibody

AMM02542G Product Datasheet Protocol

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AMM02542G		100 μl / $495

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Leading Biology Inc.

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ETS1 Antibody

Brand	Leading Biology	Catalog Number	AMM02542G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ETS1 Antibody.
Molecular Weight	50408 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27
Host	Mouse
Species Reactivity	Human
Target	This ETS1 antibody is generated from a mouse immunized with a recombinant protein of human ETS1.
Clone	1601CT512.35.61
Isotype	IgG2b,k
Symbol	EWSR2
GeneID	2113
UniProt ID	P14921
Function	Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.
Summary	Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, WB, E
Dilution	FC~~1:25 WB~~1:500
Images	Overlay histogram showing K562 cells stained with AMM02542G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM02542G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Anti-ETS1 Antibody at 1:500 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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