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General MDA/Malondialdehyde ELISA KitBrand |
Leading Biology | Catalog Number |
EK00830E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00830E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Malondialdehyde) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Malondialdehyde The ELISA analytical biochemical technique of the EK00830Ee kit is based on Malondialdehyde antibody-Malondialdehyde antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Malondialdehyde antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Malondialdehyde. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Malondialdehyde is the organic compound with the formula CH2(CHO)2. The structure of this species is more complex than this formula suggests. This reactive species occurs naturally and is a marker for oxidative stress.
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Assay Type |
Competitive
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Detection Method |
Spectrophotometrical
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Reactivity |
General
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.088nmol/mL
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Assay Range |
0.312-20nmol/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
MDA
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Function |
Malondialdehyde reacts with deoxyadenosine and deoxyguanosine in DNA, forming DNA adducts, the primary one being M1G, which is mutagenic. The guanidine group of arginine residues condense with MDA to give 2-aminopyrimidines.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Images |
Standard curve of the product EK00830E. |
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Specification |
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Quantity |
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