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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human IgM/Immunoglobulin M ELISA Kit

Human IgM/Immunoglobulin M ELISA Kit

Catalog # Availability Size / Price Inquiry
EK00009E 96T / $545

Human IgM/Immunoglobulin M ELISA Kit

Brand

Leading Biology

Catalog Number

EK00009E

Product Type

ELISA Kit

Field of Research

Immunology

Product Overview

EK00009E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Immunoglobulin M) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Immunoglobulin M The ELISA analytical biochemical technique of the EK00009E kit is based on Immunoglobulin M antibody-Immunoglobulin M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Immunoglobulin M antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Immunoglobulin M. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Cellular Localization

Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen.

Assay Type

Sandwich

Detection Method

Spectrophotometrical

Reactivity

Human

Sample Type

This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Sensitivity

3.8 ng/mL

Assay Range

7.8-500 ng/mL

Kit Components

Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

Other Reagents Required

Deionized or distilled water

Gene Name

IgM

Function

Due to its polymeric nature, IgM possesses high avidity, and is particularly effective at complement activation. By itself, IgM is an ineffective opsonin; however it contributes greatly to opsonization by activating complement and causing C3b to bind to the antigen.

Form

ELISA Kits

Storage & Stability

Store at +4°C. Please refer to protocols.

Applications

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Images

Standard curve of the product EK00009E.

Specification

Quantity

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