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  • Polyclonal Antibody Production
    Polyclonal Antibodies Introduction Polyclonal antibodies are antibodies which have been derived from multiple B cells or cell lines. A preparation of these antibodies bears some resemblance to the antibody variety found in normal serum, which is the fluid component that separates from clotted blood. The resemblance is due to the fact that antibodies that are polyclonal recognize different epitopes and have different degrees of specificity. In contrast, the antibodies in a monoclonal preparation are derived from one clonal type and recognize the same epitope with the same degree of specificity. To produce custom polyclonal antibodies, an animal such as a chicken, mouse or rabbit is immunized with an antigen and adjuvant preparation. The animal’s immune system is stimulated to produce B cells which secrete antibody that is specific for the antigen. After a period of time, typically several weeks or even months, the animal’s serum is harvested. Custom polyclonal antibody preparations are usually a mixture of antibody specificities which all recognize the same antigen. The specificity difference means the antibodies bin...
  • Monoclonal Antibody/Hybridoma Custom Service
    Monoclonal Antibody/Hybridoma Antibodies produced by the clones of one B-cell are called monoclonal antibodies (mAb or moAb). Monoclonal antibodies are highly specific immunoglobulin designed to target antigens. Therefore, they have been widely used in the diagnosis of disease, whether in the diagnostic laboratories, in the doctor's rooms or in the field. In addition, they are useful in designing vaccines or carrying toxins to a target cell without affecting adjacent cells. The antibodies produced by hybridoma are all of a single specificity and therefore are monoclonal antibodies. The mentioned hybridoma technology is the one forms hybrid cell lines (hybridomas) by fusing a specific antibody-producing B cell with myeloma tumor cells (B-cell cancer), which is selected for its ability to grow in tissue culture and for an absence of antibody chain synthesis. Preparation   Classic Case Publication:Acta Biochim Biophys Sin IF:2.191 Title:ACAT1 regulates the dynamics of free cholesterols in plasma ...
  • Polyclonal Antibody Serum Preparation
    Introduction Leading Biology provides fully customizable services for polyclonal antibody production ranging from peptide design and synthesis to antibody purification and detection. A variety of different hosts to produce polyclonal antibodies are used, including rabbit, rat, mouse, goat, guinea pig, chicken, sheep, monkey, etc. Each project will be discussed thoroughly before starting the protocol so as to sure that your demands will be fulfilled.  Polyclonal antibodies (pAbs) are a group of antibodies that could bind to different epitopes of the same antigen. Even if a virus or bacteria changes its structure just a little bit, the antibodies will still be able to find and attach to it, which is different from the single combination of monoclonal antibodies. That is why researchers often use polyclonal antibodies to identify parts of unknown molecules. Apart from that, the polyclonal antibody preparation is cheaper, quicker and easier in comparison to monoclonal antibody preparation. Service process and package Each cus...
  • Rabbit Monoclonal Antibody preparation by phage display
    Introduction Leading Biology dedicated itself from the first day to develop monoclonal antibodies almost exclusively. We have an onsite vivarium and working to establish a GMP facility soon. We keep a veterinarian on staff to make sure our mice and other animals are in the best health. We know how to generate an antibody that is specific to the immunizing antigen and that works for the intended application. Our technical staff with decades of experience in immunology, biochemistry, and molecular biology will be there to guide your through the 10 to 14-week process. Service Package Service Items Time(weeks) Peptide Molecular Phosphate/Methyl/ acetyl-specific Peptide design & synthesis Peptide design ~2 ...
  • Mouse Monoclonal Antibody preparation by phage display
    Introduction Leading Biology dedicated itself from the first day to develop monoclonal antibodies almost exclusively. We have an onsite vivarium and working to establish a GMP facility soon. We keep a veterinarian on staff to make sure our mice and other animals are in the best health. We know how to generate an antibody that is specific to the immunizing antigen and that works for the intended application. Our technical staff with decades of experience in immunology, biochemistry, and molecular biology will be there to guide your through the 10 to 14-week process. Service Package Time(weeks) Molecular Peptide design & synthesis ~2 √ ...
  • Antibody Labeling Services
    Custom Antibody Labeling Services When assaying antigens with an antibody, it is necessary to detect the presence of that antibody in the assay through one of two methods. The first method is to target the antibody indirectly with the use of a secondary antibody. Secondary antibodies are widely available and are coupled to labels such as FITC or Biotin and bind specifically to the primary antibody. The second method is to conjugate one of these labels directly to the primary antibody, thereby eliminating the need for the secondary antibody. Our in-house expertise enables us to provide a wide range of services, adaptable to your requests.  Examples of custom projects  Custom conjugation of Ab fragments to PEGs Custom conjugation of ovalbumin to Ab / proteins Optimization and conjugation of oligos to folate molecules Site directed conjugations Magnetic beads conjugation microoptimization and scale-up conjugation Latex beads conjugation microoptimization and scale-up conjugation Europium beads conjugation microoptimization ...
  • Custom Phosphospecific Antibody Production
    Overview Our Custom Phosphospecific Antibody Production package includes everything needed to obtain affinity-purified antibodies that are specific to a phosphorylated epitope. We run an extensive series of ELISA assays on the production bleeds and on the affinity purified antibodies to ensure that antibodies are specific to the phosphorylated peptide and show no activity against the non-phosphorylated peptide. Service Items Details Protein Sequence Analysis lSynthesis of up to a 15aa Phosphorylated Peptide l13-week Antibody Production Protocol: nPre-Immune Serum from each animal n4 Production Bleeds of 20-25 ml of serum per animal nELISA on the 1st Production Bleed against both peptides lAffinity Purification of 25 ml of serum against the phosphorylated peptide lBoth re-useable columns included l$150.00 Package Pricing Discount included Service Details ...
  • Custom Peptide Antibody Production
    Overview Our Custom Peptide Antibody Production package includes everything needed to obtain custom antibodies against a synthesized peptide. Please note that ELISA and Affinity Purification of the serum against the peptide is not included with this package. For the best results, we recommend our Monospecific Antibody Production Package, which includes these services with a package pricing discount. Affinity purification of the serum against the peptide will eliminate non-specific cross reactivity in the serum and maximize specificity of the anti-peptide antibodies in your assays. Details Protein Sequence Analysis l  Conjugation of 3-5 mg of the peptide to KLH l  13-week Antibody Production Protocol: u  Pre-Immune Serum from each animal u  4 Production Bleeds of 20-25 ml of serum per animal u  Chickens l  Synthesis of up to a 15aa peptide l  Handling / Shipment Preparation ...
  • Custom Monospecific Antibody Production
    Introduction Custom Monospecific Antibody Production is our most popular package. It can serve as a cost effective alternative to monoclonal antibody production for many applications. By immunizing with a peptide sequence and then affinity purifying the serum against peptide sequence, it is possible to isolate highly specific antibodies that approach the specificity of monoclonal antibodies but provide the superior affinity of polyclonal antibodies. Details Protein Sequence Analysis lConjugation of 3-5 mg of the peptide to KLH l2 Rabbits u4 Immunizations per animal uProvides approximately 160-200 ml of anti-sera lAffinity Purification of 25 ml of Serum from 3rd Production Bleed lHandling / Shipment Preparation Service Process Details Cost per each additional peptide residue over the 15 residues included with the package ...
  • Anti-Membrane Protein Antibody Discovery
    Anti-Membrane Protein Antibody Discovery Using Hybridoma Leading Biology has extensive experience in hybridoma technology. Our mature hybridoma platform offers both mouse and rat animal models to raise anti-GPCR antibodies. Vigorous immune response and high cell fusion efficacy can be guaranteed, followed by comprehensive screening of large candidate pools. Our team is specialized in various high throughput screening strategies against recombinant proteins, reconstituted formats, living cells, etc. Of note, we have established a novel transgenic mouse strain that can produce fully human anti-GPCR antibodies, which can greatly facilitate therapeutic/diagnostic antibody discovery. Anti-Membrane Protein Antibody Discovery by Phage Display Technology Leading Biology offers one-step phage display library construction and screening service. Equipped with this world-leading phage display platform, we have rich experience in conducting versatile bio-panning strategies, including in-solution panning, solid-phase panning, cell-based panning, in vivo panning, etc. ...
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