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  • Special Functional Antibody Service
    Introduction For different experimental uses of antibodies, to fully meet the customer's experimental needs, our technicians doing objective research for different purposes of each customer's experiment, trying to provide efficient and accurate antibody preparation technology services for customers with the most reliable qualities. we can not only undertake the entire service from the upstream antibody preparation to the downstream experimental application, but also provide preliminary test results, cooperate with customers' validation test, in every aspect of the experiment: technology discussion, test improvement and result validation, we'd communicate with customers timely and accurately to meet all kinds of requirements of scientific research, medicinal, industry, health and other fields. Classic Case Publication:Res Vet Sci.     IF:2.191 Title:Changes in the expression of Heat Shock Proteins in ovaries from bovines with cys...
  • Canine Monoclonal Antibody Service
    Introduction Canines are often used as animal models for vicious infection, inflammation and autoimmune diseases, especially some digestive system diseases. Based on rich experimental experience and technology accumulation of phage display, Leading Biology, with experience of large scale elutriation screening of high affinity dog mAb, has successfully established an immunization antibody library against several antigens. The antibodies prepared can be widely used in the biomedical field. In some cases,  due to some special reasons for the antigen itself, for example, immunogenicity or toxicity, it is difficult for the antibody to prepare the immune pathway, at this time, our knowledge accumulation plays an important role in dog antibody preparation, by construction of the antibody light and heavy chain frame structure and site modification of specific antigens, achieve the fully artificial production of antibodies. But this method involves the prediction of the spatial structure of the antigen and antibody configuration, the implement difficultly and the technical requirements are very high. In addition to canine monoc...
  • Monkey Monoclonal Antibody Service
    Introduction Different from small molecular compounds, the biotech drugs may have a strong stimulation of the specific and sensitized lymphocytes when getting into the body. The anti-drug antibodies may cause adverse reactions like anaphylaxis, immunosuppression and autoimmune response of the body. The monkey source antibody has a higher homology with human antibody compare to other mammalian source antibodies, thus the study of immunology and pharmacology carried out in monkeys has higher data reliability. The anti-drug antibodies produced by monkeys for certain drugs usually could simulate the same reactions in the human body. Based on phage display technology, we could achieve rhesus, macaque, marmoset and ateles immune organs cDNA library full spectrum construction and screening, the synthesized antibody library can guarantee high detection rates and screening ranges. At the same time, the antibody specificity and titer are higher than other rodents or rabbits antibodies. Leading Biology has a complete antibody humanization technical service, could provide technical support for further processing of human recombinant antibodies. S...
  • Chicken Monoclonal Antibody Service
    Introduction  Compared with common mammalian IgG, the chicken IgY antibody has a strong resistance to heat, acid, ionic and have certain enzyme resistance, it can be used as an ideal diagnostic reagent and a drug for prevention and treatment. The Leading Biology using SPF level chicken, ensures the pure background and higher production of the antibody, by using international advanced nano latex immuno amplification technology, acquire antigens with stronger immunogenicity, improve the immunization success possibility, and get chicken antibody with higher titer. Service Package Service Items Peptide Phosphate/Methyl/Acetyl-specific    √   √   Immunization Immune 2 chickens -8 √ ...
  • Rabbit Monoclonal Antibody Production
    Introduction There are two sets of preparation schemes for the preparation of rabbit monoclonal antibody. Both schemes do not depend on the rabbit myeloma cells, but the provenance of the antibodies is undoubtedly rabbit, these methods greatly shorten the test time and improve the success rate, while preserving the advantage of the rabbit monoclonal antibody. Plan A: Phage Display The M13 phage display system is a powerful technology for engineering proteins such as functional mutant proteins and peptides, by expression protein/antibody/polypeptides-phage pIII protein fusion, the target protein was involved in phage assembly and displayed on the phage surface to form a phage display library. Using phage display library to targeting the specific target molecules for 3-5 rounds of affinity screening, we can collect phages that specifically bind the target molecules, and get relevant DNA sequence information. The phage display technology service platform designed by Leading Biology including common libraries, such as the immune antibody library, natural antibody library, etc. Can customizing personalized library according to the special needs of c...
  • Hamster Hybridoma Production
    Introduction Together with Diagnostics, Leading Biology has extensive experience in producing monoclonal antibodies in hamsters. The use of hamsters as the host animal offers a unique species alternative in areas where mouse and rat antibodies may not work well. We can employ up to 10 hamsters for each antigen. Immune responses are elicited using immunogens such as transfected cell lines, DNA, recombinant proteins, native proteins, peptides or haptens conjugated to carrier molecules. We perform splenocyte fusion on one or more animals. We select and subclone the strongly positive clones from the parental cultures by 2 rounds of limiting dilution cloning. Stable clones will be expanded into 24-well plates and then into T-flasks for further characterization. At no additional cost, clients could select one clone for in vitro production of approximately 1L of supernatant. Supernatants will be purified using Protein A/G column and pure antibodies will be deliverable. We also offer a full range of contract manufacturing services, including in vitro Antibody and Protein Production in bioreactors and large-scale Antibody Purification. ...
  • Rat Monoclonal Antibody Service
    Introduction Leading Biology has established a unique platform to develop high-affinity monoclonal antibodies in rats. Compared with commonly used mouse antibodies, rat antibodies do not have the background cross-reaction problems in immune detection of antigens out of a mouse background, such as a mouse antigen from a mouse animal model. However, Rat monoclonal antibodies are much more difficult to develop compared with mouse monoclonal antibodies, due to the lack of a stable fusion partner cell line for rat hybridoma generation.   Leading Biology has developed a proprietary hybridoma-generation platform for efficient development of high-affinity and high-specificity rat monoclonal antibodies. Our Rat Monoclonal Antibody platform allows us to offer the most comprehensive one-stop custom antibody development services in the industry. Service Package Service Items (months) Peptide Small Molecule Phosphate/Methyl/Acetyl-...
  • Mouse Monoclonal Antibody Service
    Introduction Leading Biology dedicated itself from the first day to develop monoclonal antibodies almost exclusively. We have an onsite vivarium and working to establish a GMP facility soon. We keep a veterinarian on staff to make sure our mice and other animals are in the best health. We know how to generate an antibody that is specific to the immunizing antigen and that works for the intended application. Our technical staff with decades of experience in immunology, biochemistry, and molecular biology will be there to guide you through the 10 to 14-week process. Service Package SmallMolecule Purified protein Prokaryotic proteins synthesis de novo Peptide synthesis       Protein preparation ...
  • Rapid Antibody Service
    Introduction It is possible to produce usable antibodies within a short time, when the peptide is designed and produced by us, we guarantee reactivity of the immune serum with the peptide. As with any antibody project there is no telling beforehand how well the antibody will work in your application, if at all. It may be that all that is required is more time for the antibodies to mature. If you don't have the antigen readily available, you could use a synthetic peptide conjugated to a carrier molecule as the immunogen. Proper peptide design work and the use of high quality reagents and a robust synthesis protocol will allow for a rushed peptide production, which shields your project from serious delays while ensuring that quality is maintained. Accelerated immunization protocols may be called for in special cases. However, the vast majority of custom antibody projects are not special in that respect, but rather the opposite. Instead of accelerating the booster intervals one should apply a standard protocol that is tested and proven for thousands of projects, and make adaptations so that earlier bleeds are accommodated. You w...
  • Chicken Polyclonal Antibody Service
    Introduction Compared with common mammalian IgG, chicken IgY antibody has a strong resistance to heat, acid, ionic and have certain enzyme resistance, it can be used as an ideal diagnostic reagent and a drug for prevention and treatment. Leading Biology using SPF level chicken, ensures the pure background and higher production of the antibody, by using international advanced nano latex Immuno amplification technology, acquire antigen with stronger immunogenicity, improve the immunization success possibility, and get chicken antibody with higher titer. Service Package  (at least 5 chickens) Service Items (weeks) Protein package Peptide design 2-3 Free, Optional     √       Prokaryotic...
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