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Location: Home > Custom Services > Protein Related Services > Protein Expression in Bacteria

Protein Expression in Bacteria

Date: 2019-03-31 Author: Leading Biology Click: 11609

Introduction Protein Expression in Bacteria

Bacterial expression system is an ideal protein expression system of all time because of its nature. It often acts as the first choice in producing recombinant proteins and antibodies. Many bacteria have already been well characterized and investigated until now.


Among the huge number of bacteria, E. coli and B. subtilis cell lines are the most commonly used hosts for recombinant protein expression. They replicate extremely fast under the right condition at a low cost, while the expression level is high, which makes it an efficient and economical way for large scale production. Their genomes have been entirely sequenced and the genetic backgrounds and metabolic pathways are well known. Additionally, because of the simple structures, bacteria are more easily transfected than eukaryotes. These desirable characteristics make the expression procedure easier and quicker.


But every coin has two sides. Proteins with few modifications could be expressed in bacteria easily. While there is no nucleus, Golgi apparatus, and endoplasmic reticulum in bacteria. These structures are necessary in the process of cellular transport and post-translational modifications (PTMs). This leads to the incapability to produce membrane-bound proteins with bacterial expression systems, including glycophorins, several kinds of enzymes, and so on. Since such modifications are required in the correct folding, proteins produced in bacteria are often inactive and not functional. In terms of these respects, the eukaryotic expression system is more suitable to express these proteins that need folding, modification, and special structures, even if it is more expensive and takes more time than the bacterial expression system. Each expression system has its merits and demerits. 


Leading Biology team will fully evaluate each project and recommend the most suitable system according to customers’ requirements.


Company profile

Leading Biology Inc. is an innovative high-tech company that produces bio-products (antibodies, proteins, cells, and chemicals) for a wide range of research and medical fields. We provide customers with high quality services under the support of our professional technical team with decades of experience in the biotechnology field. Our core values of honesty, integrity and respect for customers define who we are and how we work. We are dedicated to providing the best quality, most efficient, and professional services to our customers.



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Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.


  • Innovative configurations of chromatography columns custom-tailored to each protein
  • Vigorous quality control system to ensure the required quality and reproducibility
  • Production capacity of up to tens of grams
  • Flexible scale-up protein production
  • Competitive price with fast turnaround time


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

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By Phone: 1-661-524(LBI)-0262 (USA)

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