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Rich in Disulfide Bond Protein ExpressionService introduction
As we all know, the structure of the protein which rich in the disulfide bond is relatively complex, the solubility of the target protein expressed by the prokaryotic system is quite low, and is very easy to form an inclusion body, thus, the probability of getting the active protein is very low.
Leading Biology using a new prokaryotic expression system, greatly increased the probability of disulfide bond rich protein expression in Escherichia coli, the expression rate is greater than 90%.
Service process
Service Items |
Time(weeks) |
Protein analysis and codon optimization |
Before project start |
Gene synthesis |
2-4 |
Vector construction |
1 |
E.coli expression strains transformation and screening |
2 |
Target protein inducible expression |
1 |
Target protein purification and detection |
2 |
Large scale fermentation and purification |
According to the requirements of the project |
Total |
8-10 |
Requirements of the samples provided by customers
1. Gene sequence or amino acid sequence of the target protein.
2. If the vector is prepared, the detailed information of the vector is required.
Advantages of Leading Biology
1. Rich experience of protein expression;
2. Well-designed multi-technology platform;
3. Leading Biology could provide a complete technical service process, from upstream gene synthesis, vector construction to downstream protein purification, and functional verification. We also could take over the project from any step according to the requirements of the customers.
Product
1. Provide sufficient protein samples as required by the customers;
2. A complete report of technical services.
Related Services
Protein Expression in Bacteria
Unique Protein Production Service
Protein Expression in Cell-Free System
Rich in Disulfide Bond Protein Expression
Protein Expression in Mammalian Cells
Protein Expression in Baculovirus
Protein Expression in Bacillus subtilis
Why Leading Biology?
At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.
Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.
Working with us, you will get the guaranteed service to accommodate your requirements.
- Innovative configurations of chromatography columns custom tailored to each protein
- Vigorous quality control system to ensure the required quality and reproducibility
- Production capacity of up to tens of grams
- Flexible scale-up protein production
- Competitive price with fast turnaround time
Contact Information
Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)
No | Headline | Click | Author | Date |
1 | Ubiquitination | 1208 | Leading Biology | 2020-06-24 |
2 | Acetylation | 776 | Leading Biology | 2020-06-23 |
3 | Single Molecule Protein Detection | 1021 | Leading Biology | 2020-06-22 |
4 | Phosphorylation | 1045 | Leading Biology | 2020-06-22 |
5 | Methylation | 1122 | Leading Biology | 2020-06-22 |
6 | Protein Detection and Immunoassay | 1154 | Leading Biology | 2020-06-17 |