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Location: Home > Custom Services > Protein Related Services > Rich in Disulfide Bond Protein Expression

Rich in Disulfide Bond Protein Expression

Date: 2018-08-28 Author: Leading Biology Click: 2372

Service introduction

As we all know, the structure of the protein which rich in the disulfide bond is relatively complex, the solubility of the target protein expressed by the prokaryotic system is quite low, and is very easy to form an inclusion body, thus, the probability of getting the active protein is very low.

Leading Biology using a new prokaryotic expression system, greatly increased the probability of disulfide bond rich protein expression in Escherichia coli, the expression rate is greater than 90%.


Service process

Service Items

Time(weeks)

Protein analysis

and codon optimization

Before project start

Gene synthesis

2-4

Vector construction

1

E.coli expression strains transformation and screening

2

Target protein

inducible expression

1

Target protein purification and detection

2

Large scale fermentation and purification

According to the requirements of the project

Total

8-10



Requirements of the samples provided by customers

1. Gene sequence or amino acid sequence of the target protein.

2. If the vector is prepared, the detailed information of the vector is required.


Advantages of Leading Biology

1. Rich experience of protein expression;

2. Well-designed multi-technology platform;

3. Leading Biology could provide a complete technical service process, from upstream gene synthesis, vector construction to downstream protein purification, and functional verification. We also could take over the project from any step according to the requirements of the customers.


Product

1. Provide sufficient protein samples as required by the customers;

2. A complete report of technical services.



Related Services

Protein Expression in Bacteria

Unique Protein Production Service

Protein Expression in Cell-Free System

Rich in Disulfide Bond Protein Expression

Protein Expression in Mammalian Cells

Protein Expression in Baculovirus

Protein Expression in Yeast

Protein Expression in Bacillus subtilis

Protein Expression in E coli

Virus-like particles/Phage library expression system based

Cell-free expression system

Whole cell-based protein expression system


Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.

  • Innovative configurations of chromatography columns custom tailored to each protein
  • Vigorous quality control system to ensure the required quality and reproducibility
  • Production capacity of up to tens of grams
  • Flexible scale-up protein production
  • Competitive price with fast turnaround time

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

Order Products: Order Related Products

By Phone: 1-661-524(LBI)-0262 (USA)



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No Headline Click Author Date
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