Introduction

At the initial of mammalian immune process, antigens are not directly processed by Th cells, but processed and presented by the antigen-presenting cells (APC). If we can produce cells that express target antigens, these antigens will be expressed on the cell surface, this kind of cell can be used to immunize animals as antigens.

The advantages of this method are: in situ expression of transmembrane protein in eukaryotic system ensures its complete post-translational modification process, meanwhile, the natural structural characteristics are well preserved by using the cell membrane as they assemble and display surface.

However, it also has many disadvantages: since the cell is too large compared to the target antigen, and the surface also contains some modificated non-target antigens, so as an antigen, although the immunogenicity can be guaranteed, the validity and specificity are not. The solution of this problem including high expression of target antigen and a large number of screening to identify and exclude antibodies which recognize invalid antigens.

Service Package

Why Leading Biology?

We offer the one-stop phage display service:

·      Immune, naive or synthetic library construction

·      Pre-made library screening

·      Monoclonal detection

·      Antibody expression

·      Custom biopanning strategies

·      QC: to ensure the success of your projects, we perform documentation QC for all assays.

Our advantages

·      World-leading sequencing capacity: use state-of-the-art technologies, such as Timer Condon technology, nanopore platform, etc with library capacity from 10⁸ to over 10¹³ ;

·      Various phage display systems: provide the most appropriate phage display system (M13, T4 or T7) to meet our customers’ demands.

·       High affinity: from 10^-7 to 10^-9

·      Custom biopanning strategies: The experienced technical team will work together to customize our products so as to meet our customers’ different needs. Leading Biology also utilizes the novel biopanning strategies to select high-affinity binders or ligands which are able to recognize the naive targets. Our Naive human antibody library panning includes: Pre-made yeast display naive human antibody library & Pre-made phage display naive human Fab library.

·      Rich experience: Our technicians refine every detail of our processing to enable the efficiency and high-quality. With extensive experience in phage display, we have provided satisfying service to our global researchers.

Contact US

We are proud to be able to support global scientists by providing high-quality service in a rapid time-frame. Reach out to us and we will get back to you within 24 hours.

Get a free quote

Email: info@leadingbiology.com

Phone: 1-661-524-0262 (USA)