Introduction
The SNP array is a kind of DNA microarray that constitutes a powerful tool for high-throughput analysis of thousands of SNPs in a single experiment to globally analyze the human genome for genetic alteration. The basic principles of SNP array are the same as the DNA microarray. These are the convergence of DNA hybridization, fluorescence microscopy, and solid surface DNA capture. The three mandatory components of the SNP arrays are: (1). An array containing immobilized allele-specific oligonucleotide (ASO) probes; (2). Fragmented nucleic acid sequences of target, labeled with fluorescent dyes; and (3). A detection system that records and interprets the hybridization signal.
SNP chips are generally described by the number of SNP positions they assay. Two probes must be used for each SNP position to detect both alleles; if only one probe were used, experimental failure would be indistinguishable from homozygosity of the non-probed allele.
No | Headline | Click | Author | Date |
1 | Plant and Animal Whole Genome Re-Sequencing | 829 | Leading Biology | 2018-01-26 |
2 | Whole Exome Sequencing | 820 | Leading Biology | 2018-01-26 |
3 | Whole Transcriptome Shotgun Sequencing | 1126 | Leading Biology | 2018-01-26 |
4 | smallRNA/microRNA/circRNA/LncRNA Sequencing | 805 | Leading Biology | 2018-01-26 |
5 | Bacterial Genome Sequencing | 815 | Leading Biology | 2018-01-26 |
6 | Targeted Gene Sequencing | 912 | Leading Biology | 2018-01-26 |