Introduction
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system is a prokaryotic immune system that confers resistance to foreign genetic elements such as those present within plasmids and phages that provides a form of acquired immunity. RNA harboring the spacer sequence helps Cas (CRISPR-associated) proteins recognize and cut exogenous DNA. These sequences play a key role in a bacterial defense system, and form the basis of a technology known as CRISPR/Cas9 that effectively and specifically changes genes within organisms.
CRISPR-Cas9 is a unique technology that enables geneticists and medical researchers to edit parts of the genome by removing, adding or altering sections of the DNA sequence. It is currently the simplest, most versatile and precise method of genetic manipulation.
The CRISPR-Cas9 system consists of two key molecules that introduce a mutation into the DNA: Cas9 and gRNA.
Cas9 acts as a pair of ‘molecular scissors’ that can cut the two strands of DNA at a specific location in the genome so that bits of DNA can then be added or removed. While the guide RNA (gRNA) is a small piece of pre-designed RNA sequence located within a longer RNA scaffold. The scaffold part binds to DNA and the pre-designed sequence ‘guides’ Cas9 to the right part of the genome. This makes sure that the Cas9 enzyme cuts at the right point in the genome.
Why Leading Biology?
At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.
Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.
Working with us, you will get the guaranteed service to accommodate your requirements.
· Vigorous quality control system to ensure the required quality and reproducibility
· Competitive price with fast turnaround time
Contact Information
Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)
No | Headline | Click | Author | Date |
1 | siRNA/shRNA gene knockdown | 1243 | Leading Biology | 2018-01-26 |
2 | Tetracycline Induced Gene knockout/knockin | 1570 | Leading Biology | 2018-01-26 |
3 | New Generation Embryonic Stem Cells Gene Targeting | 1575 | Leading Biology | 2018-01-26 |
4 | TALEN Technology | 1485 | Leading Biology | 2018-01-26 |
5 | CRISPR-CAS Technology | 1600 | Leading Biology | 2018-01-26 |
6 | Microorganisms Gene Modification Services | 1366 | Leading Biology | 2018-01-26 |