Introduction

Chemiluminescence is the emission of cold light as a result of a chemical reaction. When a chemical reaction occurs, the reactions either give off (exothermic) or absorb (endothermic) heat, but there are few kinds of chemical reactions in which the energy produced is given off not as heat but as light, that is chemiluminescence.

Chemiluminescent Assay is an important technique in molecular biology. There are two ways in which the chemiluminescent assay used in the lab: the one-step sandwich technique and the two-step immunocapture technique. The procedures are like below:

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