Introduction

Immunocytochemistry (ICC) is a common laboratory assay that can confirm the expression and location of target peptides or protein antigens in the cell via specific combination of antibodies and target molecules, It offers a semi-quantitative means of analyzing the relative abundance, conformation and subcellular localization of target antigens.

There are many methods to obtain immunological detection on tissues, traditional ICC techniques use chromogenic detection in which enzyme conjugated antibodies convert chromogen substrates to a colored precipitate at the reaction site, immunofluorescent labels are also used to show the reaction site. The cellular antigen was visualized using either fluorochrome-conjugated primary antibodies (direct detection) or a two-step method (indirect detection) involving an unlabeled primary antibody followed by a fluorochrome-conjugated secondary antibody. 

The ICC protocol including fixation, permeabilization, blocking, immunolabeling, counterstaining and microscopic imaging. The process is like below:

3. Analysis:

Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

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