Introduction

Co-Immunopericpitation (Co-IP) is one of the most straightforward technique to study protein-protein interactions in vivo thereby elucidating signaling pathways. Same to Immunoprecipitation assay, Co-IP method also involves incubating specific antibody along with the protein of interest to form an immune complex. This complex is then precipitated onto an immobilized support, ex: antibody-coupled beads, and then wash to remove the unbound protein. Protein components in the complexes are then visualized by Western blot and analyzed by SDS-PAGE. However, there are some difference between Co-IP and IP, Co-Immunoprecipitation is more focused on the additional molecules that are bound to the target protein by inherent interactions in the sample complex.

5. Analyse samples by SDS-PAGE and Western blot.

Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

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