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Polyclonal Antibody Serum PreparationService process and packageLeading Biology provides fully customizable services for polyclonal antibody production ranging from peptide design and synthesis to antibody purification and detection. A variety of different hosts to produce polyclonal antibodies are used, including rabbit, rat, mouse, goat, guinea pig, chicken, sheep, monkey, etc. Each project will be discussed thoroughly before starting the protocol so as to sure that your demands will be fulfilled.
Polyclonal antibodies (pAbs) are a group of antibodies that could bind to different epitopes of the same antigen. Even if a virus or bacteria changes its structure just a little bit, the antibodies will still be able to find and attach to it, which is different from the single combination of monoclonal antibodies.
That is why researchers often use polyclonal antibodies to identify parts of unknown molecules. Apart from that, the polyclonal antibody preparation is cheaper, quicker and easier in comparison to monoclonal antibody preparation.
Classic Case
Each custom service will be paid unique attention with details so that you can achieve the next biotechnology breakthrough. Also, you will receive updates on project status so you can better know about the process.
Service details1
Publication:Nature(letters)
IF:41.456
Title:A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defense
Name:Anti-FLS2 antibody
Type:Rabbit polyclonal antibody
Species: Arabidopsis thaliana
2
Publication:Cancer Science
IF:3.054
Title:Phloroglucinol suppresses the metastatic ability of breast cancer cells by inhibition of epithelial-mesenchymal cell transition
Name:FN1; mouse polyclonal antibody
Type:Mouse polyclonal antibody
Species: Human Fibronectin
3
Publication:Tumor and Stem Cell Biology
IF:5.013
Title:MicroRNAs Regulate Tumor Angiogenesis Modulated by Endothelial Progenitor Cells Name:Rat anti-mouse miR-10b/miR-196b
Type:Rat monoclonal antibody
Species: Mouse
4
Publication:Plos One
IF:3.05
Title:Phosphodiesterase Type 4 Inhibitor Rolipram Improves Survival of Spiral Ganglion Neurons In Vitro
Name:Anti-200kD Neurofilament antibody
Species: Mouse
5
Type:Chicken polyclonal antibody
Publication:FEBS letters
IF:3.169
Title:Multiple FtsZ2 isoforms involved in chloroplast division and biogenesis are developmentally associated with thylakoid membranes in Arabidopsis
Name:Anti-FtsZ2 antibody
Type:Rabbit polyclonal antibody
Species: Arabidopsis thaliana
Antigen Forms
Polyclonal antibodies can be generated against both soluble and non-soluble recombinant proteins, fusion proteins, gel strips, existing peptide, etc. Live or toxic substances cannot be accepted.
Antigen Quantity
We recommend sending 2-3 mg of the antigen for a rabbit or chicken project and 5-7 mg for a goat project. Extensions or immunochemistry services may require additional antigen.
Antigen Concentration
Leading Biology can work with most protein concentrations as long as this information is provided. 1 mg/ml is a good target range, but higher concentrations are welcome. We consider 0.25 to .5 mg/ml a minimum concentration in most cases to ensure maximum protein volumes in each immunization.
Considerations for Gel Bands
Immunizing with gel bands works well for generating polyclonal antibodies against a purified protein. If providing a gel strip, please stain with coomassie blue, de-stain, rinse and cut out the strip containing the target protein. Please avoid freezing or homogenizing the gel strip so that we can aliquot appropriate quantities of the protein for each immunization. With gel bands, in particular, it is important to maintain a higher concentration of the protein as outlined above.
Shipping Antigens to Leading Biology
Please ship the antigen in a Styrofoam box with ice packs (dry ice isn’t necessary) via FedEx, UPS or DHL Overnight to the address listed on our Order Form.
Related Services
Why Leading Biology?Rapid Antibody Service
Chicken Polyclonal Antibody Service
Rat Polyclonal Antibody Service
Mouse Polyclonal Antibody Service
Contact Information
With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:
• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.
• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.
Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)
No | Headline | Click | Author | Date |
1 | Antibody Production | 2540 | Leading Biology | 2020-01-16 |
2 | Mouse Monoclonal Antibody preparation by phage display | 1315 | Leading Biology | 2019-11-20 |
3 | Rabbit Monoclonal Antibody preparation by phage display | 1695 | Leading Biology | 2019-11-20 |
4 | Antibody Labeling Services | 5731 | Leading Biology | 2019-11-05 |
5 | Custom Monospecific Antibody Production | 1985 | Leading Biology | 2019-11-04 |
6 | Custom Peptide Antibody Production | 6602 | Leading Biology | 2019-11-04 |